| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jones, Emily L (10)
|
MDHOCO |
|
42.06 |
- |
| 2 |
Garner, Luna E (10)
|
MDCAC |
|
45.90 |
- |
| 3 |
Ma, Michelle Y (10)
|
MDHOCO |
50.05 |
49.37 |
-0.68 |
| 4 |
Karunwi, Aderinsola O (10)
|
MDHOCO |
|
52.42 |
- |
| 5 |
Kuck, Madison A (9)
|
MDHOCO |
|
54.02 |
- |
| 6 |
Brinski, Rebekah * (9)
|
MDCAC |
|
55.52 |
- |
| 7 |
Van Ert, Bella M (9)
|
MDCAC |
|
56.33 |
- |
| 8 |
Cortes, Chrystelle Aice B (10)
|
MDCAC |
|
57.73 |
- |
| 9 |
Denison, Abigail F (9)
|
MDCAC |
|
1:02.61 |
- |
| 10 |
Wain, Ellie G (9)
|
MDHOCO |
|
1:03.50 |
- |
|
Arias, Mia F (10)
|
MDCAC |
|
DQ |
- |
|
Eversole, Jayden M (10)
|
MDCAC |
|
DQ |
- |
|
Mulugeta, Hanna S (10)
|
MDHOCO |
|
DQ |
- |
|
Fairfax, Reese A (9)
|
MDCBAC |
55.30 |
NS |
- |
|
Lederman, Elena R (10)
|
MDHOCO |
56.70 |
NS |
- |